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PEMT Antibody (N-term)

APR09036G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR09036G		100 μl / ￥4950

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PEMT Antibody (N-term)

品牌	Leading Biology	货号	APR09036G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PEMT antibody (N-term).
分子量	22134 Da
细胞定位	Antigen Cellular Localization: Isoform 1: Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. Note=Found in endoplasmic reticulum where most PEMT activity is generated and in mitochondria.
宿主	Rabbit
种属反应性	Human, Mouse
免疫原	1-30 aa
靶点	This PEMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PEMT.
亚型	Rabbit Ig
通用名	PEMPT, PNMT
基因ID	10400
UniProt ID	Q9UBM1
功能	Catalyzes three sequential methylation reactions of phosphatidylethanolamine (PE) by AdoMet, thereby producing phosphatidylcholine (PC).
总结	This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Alternate splicing of this gene results in three transcript variants encoding two different isoforms.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, IHC-P, E
稀释方法	WB~~1:1000 IHC-P~~1:10~50
别名	Phosphatidylethanolamine N-methyltransferase, PEAMT, PEMT, PEMT2, PEMT, PEMPT, PNMT
图像	Western blot analysis of lysates from CEM, rat L6 cell line and human liver tissue lysate(from left to right), using PEMT Antibody (N-term)( APR09036G). APR09036G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane. Western blot analysis of anti-PEMT Antibody (N-term) Pab (Cat. APR09036G) in mouse kidney tissue lysates (35ug/lane). PEMT(arrow) was detected using the purified Pab. Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PEMT antibody (N-term) (Cat. APR09036G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
说明书	Product Datasheet Protocol
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