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当前位置:首页 > 抗原抗体、ELISA、WB > 一抗 > Polyclonal Antibodies > NNMT Antibody (Center)

NNMT Antibody (Center)

货号 货期 规格 / 价格 询价
APR08760G 100 μl / ¥4950

NNMT Antibody (Center)

品牌

Leading Biology

货号

APR08760G

产品分类

Polyclonal Antibodies

研究领域

产品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality NNMT antibody (Center).

分子量

29574 Da

细胞定位

Antigen Cellular Localization: Cytoplasm.

宿主

Rabbit

种属反应性

Human

免疫原

77-106 aa

靶点

This NNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the Central region of human NNMT.

亚型

Rabbit Ig

基因ID

UniProt ID

功能

Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.

总结

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. NNMT is the protein responsible for this enzymatic activity, which uses S-adenosyl methionine as the methyl donor.

形式

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

储存条件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

应用

WB, IHC-P, E

稀释方法

WB~~1:1000 IHC-P~~1:10~50

别名

Nicotinamide N-methyltransferase, NNMT

图像

All lanes : Anti-NNMT Antibody (Center) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of NNMT (arrow) using rabbit polyclonal NNMT Antibody (Center)( APR08760G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NNMT gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with NNMT antibody (Center) ( APR08760G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

说明书

数量

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