品牌 |
Leading Biology | 货号 |
APR08952G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PCK2 antibody (N-term).
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分子量 |
70730 Da
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细胞定位 |
Antigen Cellular Localization:
Mitochondrion.
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宿主 |
Rabbit
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种属反应性 |
Human
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免疫原 |
24-54 aa
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靶点 |
This PCK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human PCK2.
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亚型 |
Rabbit Ig
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通用名 |
PEPCK2
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基因ID |
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UniProt ID |
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功能 |
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
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总结 |
This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IHC-P, IF, E
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稀释方法 |
WB~~1:1000
IHC-P~~1:50~100
IF~~1:10~50
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别名 |
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial, PEPCK-M, PCK2, PEPCK2
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图像 |
Western blot analysis of anti-PCK2 Antibody (N-term) (Cat. APR08952G) in HepG2 cell line lysates (35ug/lane). PCK2 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of PCK2 (arrow) using rabbit polyclonal PCK2 Antibody (Q39) (Cat. APR08952G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCK2 gene. |
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说明书 |
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数量 |
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