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PCK1 Antibody (C-term)

APR07004G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR07004G		100 μl / ￥4950

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PCK1 Antibody (C-term)

品牌	Leading Biology	货号	APR07004G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PCK1 antibody (C-term).
分子量	69195 Da
细胞定位	Antigen Cellular Localization: Cytoplasm.
宿主	Rabbit
种属反应性	Human, Mouse, Rat
免疫原	592-622 aa
靶点	This PCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 592-622 amino acids from the C-terminal region of human PCK1.
亚型	Rabbit Ig
通用名	PEPCK1
基因ID	5105
UniProt ID	P35558
功能	Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
总结	This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, IHC-P, IF, E
稀释方法	WB~~1:1000 IF~~1:10~50 IHC-P~~1:50~100
别名	Phosphoenolpyruvate carboxykinase, cytosolic [GTP], PEPCK-C, PCK1, PEPCK1
图像	Western blot analysis of PCK1 Antibody (C-term) (Cat. APR07004G) in rat primary hepatocyte cell line lysates. PCK1 (arrow) was detected using the purified Pab. PCK1 Antibody (C-term) (Cat. APR07004G) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PCK1 antibody detected the PCK1 protein (arrow). Confocal immunofluorescent analysis of PCK1 Antibody (C-term)( APR07004G) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
说明书	Product Datasheet Protocol
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