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当前位置:首页 > 抗原抗体、ELISA、WB > 一抗 > Polyclonal Antibodies > NOS2A Antibody (Center)

NOS2A Antibody (Center)

货号 货期 规格 / 价格 询价
APR08774G 100 μl / ¥4950

NOS2A Antibody (Center)

品牌

Leading Biology

货号

APR08774G

产品分类

Polyclonal Antibodies

研究领域

产品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality NOS2A antibody (Center).

分子量

131117 Da

宿主

Rabbit

种属反应性

Human

免疫原

830-860 aa

靶点

This NOS2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 830-860 amino acids from the Central region of human NOS2A.

亚型

Rabbit Ig

通用名

NOS2A

基因ID

UniProt ID

功能

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

总结

Nitric oxide is a reactive free radical which acts as abiologic mediator in several processes, including neurotransmissionand antimicrobial and antitumoral activities. This gene encodes anitric oxide synthase which is expressed in liver and is inducibleby a combination of lipopolysaccharide and certain cytokines. Threerelated pseudogenes are located within the Smith-Magenis syndromeregion on chromosome 17.

形式

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

储存条件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

应用

IF, WB, IHC-P, FC, E

稀释方法

IF~~1:25 FC~~1:25 IHC-P~~1:50~100 WB~~1:1000

别名

Nitric oxide synthase, inducible, Hepatocyte NOS, HEP-NOS, Inducible NO synthase, Inducible NOS, iNOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, NOS2, NOS2A

图像

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling Pdx1 with APR08774G at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

Overlay histogram showing Jurkat cells stained with APR08774G (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR08774G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

APR08774G staining NOS2A in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

说明书

数量

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