品牌 |
Leading Biology | 货号 |
APR05769G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CTSF Antibody (Center D276).
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分子量 |
53366 Da
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细胞定位 |
Antigen Cellular Localization:
Lysosome.
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宿主 |
Rabbit
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种属反应性 |
Human
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免疫原 |
261-290 aa
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靶点 |
This CTSF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-290 amino acids from the Central region of human CTSF.
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亚型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
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总结 |
Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF is a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IHC-P, FC, E
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稀释方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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图像 |
Western blot analysis of CTSF Antibody (Center D276) (Cat. APR05769G) in MDA-MB231 cell line lysates (35ug/lane). CTSF (arrow) was detected using the purified Pab.
CTSF Antibody (Center D276) (Cat. APR05769G) IHC analysis in formalin fixed and paraffin embedded human Skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CTSF Antibody (Center D276) for immunohistochemistry. Clinical relevance has not been evaluated.
CTSF Antibody (Center D276) (Cat. APR05769G) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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说明书 |
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数量 |
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