品牌 |
Leading Biology | 货号 |
APR05768G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality SPP1 Antibody (C-term).
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分子量 |
35423 Da
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细胞定位 |
Antigen Cellular Localization:
Secreted.
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宿主 |
Rabbit
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种属反应性 |
Human
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免疫原 |
273-301 aa
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靶点 |
This SPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-301 amino acids from the C-terminal region of human SPP1.
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亚型 |
Rabbit Ig
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通用名 |
BNSP, OPN
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基因ID |
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UniProt ID |
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功能 |
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
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总结 |
SPP1 binds tightly to hydroxyapatite. The protein appears to form an integral part of the mineralized matrix and probably important to cell-matrix interaction. It acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IHC-P, FC, E
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稀释方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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图像 |
Western blot analysis of SPP1 Antibody (C-term) (Cat. APR05768G) in NCI-H460 cell line lysates (35ug/lane). SPP1 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human lung carcinoma with SPP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of NCI-H292 cells using SPP1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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说明书 |
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数量 |
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