品牌 |
Leading Biology | 货号 |
APR05371G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality HSP90B Antibody (Ab-254) .
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分子量 |
84 KDa
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细胞定位 |
Antigen Cellular Localization:
Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
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宿主 |
Rabbit
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种属反应性 |
Human, Mouse, Rat
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免疫原 |
243-272 aa
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通用名 |
HSP90B, HSPC2, HSPCB
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基因ID |
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UniProt ID |
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功能 |
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
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总结 |
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IF, IHC
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稀释方法 |
WB~~ 1:1000
IF~~1:100
IHC~~1:50-1:100
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图像 |
Western blot analysis of lysates from Hela,A431 cell line (from left to right),using HSP90B Antibody (Ab-254) (B0013). B0013 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
Western blot analysis of extracts from CoLo cells (Lane 1), HepG2 cells (Lane 2) and 293 cells (Lane 3), using HSP90B (Ab-254) Antibody. The lane on the left is treated with synthesized peptide.
Immunofluorescence analysis of HeLa cells, treated with TNF-a (20nM, 15mins), using HSP90B (Ab-254) antibody . |
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说明书 |
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数量 |
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