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IGF2 Antibody (Center R54)

APR03540G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR03540G		100 μl / ￥4950

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安诺伦（北京）生物科技有限公司

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IGF2 Antibody (Center R54)

品牌	Leading Biology	货号	APR03540G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality IGF2 Antibody (Center R54).
分子量	20140 Da
细胞定位	Antigen Cellular Localization: Secreted.
宿主	Rabbit
种属反应性	Human, Mouse
免疫原	39-68 aa
靶点	This IGF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the Central region of human IGF2.
亚型	Rabbit Ig
基因ID	3481
UniProt ID	P01344
功能	The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
总结	This gene encodes a member of the insulin family ofpolypeptide growth factors, which are involved in development andgrowth. It is an imprinted gene, expressed only from the paternalallele, and epigenetic changes at this locus are associated withWilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, andSilver-Russell syndrome. A read-through INS-IGF2 gene exists, whose5' region overlaps the INS gene and the 3' region overlaps thisgene. Alternatively spliced transcript variants encoding differentisoforms have been found for this gene.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	FC, WB, IHC-P, E
稀释方法	FC~~1:25 WB~~1:1000 IHC-P~~1:50~100
图像	Overlay histogram showing Hela cells stained with APR03540G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR03540G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. All lanes : Anti-IGF2 Antibody (Center R54) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Anti-IGF2 Antibody (Center R54) at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
说明书	Product Datasheet Protocol
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