Serotonin Transporter (SERT) (extracellular) Antibody

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Serotonin Transporter (SERT) (extracellular) antibody.

70172 Da

Rabbit

Human, Mouse, Rat

Peptide ??(C)EMRNEDVSEVAKD, corresponding to amino acids residues 388-400 of rat Serotonin Transporter (Accession P31652).????4th extracellular loop.

Many physiological, endocrine and behavioral functions are determined and regulated by monoamine signaling1,2. Many brain disorders such as depression, drug abuse, schizophrenia, attention deficit hyperactivity disorder (ADHD) are caused by the malfunction of monoaminergic transmission1-3. The intensity of monoaminergic signaling is determined bythe availability of the monoamine,which is in turn determined in part by its uptake from the extracellular milieu via monoamine transporters. These transporters include DAT, SERT, and NET, responsible for uptaking dopamine, serotonin (5-HT) and noradrenaline respectively, and recycling them back for release3-5.DAT, SERT and NET are members of the Na+/Cl- dependent membrane transporter family which also includes other members. These transporters consist of 12 transmembrane domains and intracellular N- and C-termini. Like its counterparts, SERT’s intracellular N- and C-terminal domains are also subject to phosphorylation and protein-protein interactions important for modulating its activity and localization6.SERTs are evidently expressed in serotonergic neurons, and are also expressed in various peripheral tissues including specialized cells of the gut, placenta, lung, blood lymphocytes and platelets6.SERT knockout mice are viable and as a consequence exhibit increased extracellular levels of 5-HT and significantly decreased levels of brain tissue 5-HT due to deficient recycling and re-accumulation of serotonin by SERT7,8. Phenotypes displayed by SERT knockout mice include stress sensitivity, obesity and various behavioral changes9Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat serotonin transporter. Anti-Serotonin Transporter (SERT) (extracellular) antibody (#AG1204) can be used in western blot analysis, immunohistochemistry, immunocytochemistry and indirect flow cytometry applicationsand has been designed to recognize SERT from human, mouse and rat samples.

Affinity purified antibody, Liquid

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

WB

WB~~1:200-1:2000 IHC~~1:400 ICC~~1:100 FC~~1:25

Sodium-dependent serotonin transporter, 5HT transporter, 5HTT, Solute carrier family 6 member 4, Slc6a4

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