品牌 |
Leading Biology | 货号 |
APR07699G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ENASE antibody (Center).
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分子量 |
H=84,43;M=83 KDa
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细胞定位 |
Antigen Cellular Localization:
Cytoplasm, cytosol.
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宿主 |
Rabbit
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种属反应性 |
Human, Mouse
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免疫原 |
326-354 aa
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靶点 |
This ENASE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-354 amino acids from the Central region of human ENASE.
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基因ID |
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UniProt ID |
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功能 |
Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol.
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总结 |
Endo-beta-N-acetylglucosaminidase (ENGase; EC 3.2.1.96) isinvolved in the processing of free oligosaccharides in thecytosol.
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形式 |
Liquid. In PBS containing 0.02% sodium azide.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
IHC-P, FC, WB
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稀释方法 |
WB~~0.25
IHC-P~~1:50~100
FC~~1:10~50
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别名 |
Cytosolic endo-beta-N-acetylglucosaminidase, ENGase, ENGASE
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图像 |
All lanes : Anti-ENASE Antibody (Center) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: mouse testis lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
ENASE Antibody (Center) (Cat. APR07699G) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENASE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
ENASE Antibody (Center) (Cat. APR07699G) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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说明书 |
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数量 |
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