品牌 |
Leading Biology | 货号 |
APR12407G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality HTR1E antibody (C-Term).
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分子量 |
H=42 KDa
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细胞定位 |
Antigen Cellular Localization:
Cell membrane; Multi- pass membrane protein
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宿主 |
Rabbit
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种属反应性 |
Human
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免疫原 |
223-258 aa
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靶点 |
This HTR1E antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 223-258 amino acids from human HTR1E.
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基因ID |
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UniProt ID |
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功能 |
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.
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总结 |
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.
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形式 |
Liquid. In PBS containing 0.02% sodium azide.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB
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稀释方法 |
IHC~~1:25
WB~~0.25
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别名 |
5-hydroxytryptamine receptor 1E, 5-HT-1E, 5-HT1E, S31, Serotonin receptor 1E, HTR1E
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图像 |
APR12407G staining HTR1E in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-HTR1E Antibody (C-Term) at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: human brain lysate Lane 3: Jurkat whole cell lysate Lane 4: SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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说明书 |
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数量 |
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