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(Mouse) Scx Antibody (N-term)

AMM08730G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
AMM08730G		100 μl / ￥4950

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(Mouse) Scx Antibody (N-term)

品牌	Leading Biology	货号	AMM08730G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality (Mouse) Scx antibody (N-term).
分子量	M=22;H=22 KDa
细胞定位	Antigen Cellular Localization: Nucleus {ECO:0000255\|PROSITE- ProRule:PRU00981}
宿主	Rabbit
种属反应性	Mouse
免疫原	69-102 aa
靶点	This mouse Scx antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 69-102 amino acids from the N-terminal region of mouse Scx.
基因ID	20289
UniProt ID	Q64124
功能	Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.
总结	Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, IHC-P
稀释方法	IHC-P~~1:25 WB~~1:1000
别名	Basic helix-loop-helix transcription factor scleraxis, Scx
图像	AMM08730G staining Scx in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-Scx Antibody (N-term) at 1:1000 dilution Lane 1: mouse lung lysates Lane 2: mouse liver lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
说明书	Product Datasheet Protocol
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