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Goat Anti-DAP3 Antibody

AMM04953G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
AMM04953G		0.1 mg / ￥4950

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安诺伦（北京）生物科技有限公司

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Goat Anti-DAP3 Antibody

品牌	Leading Biology	货号	AMM04953G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Goat Anti-DAP3 antibody.
分子量	45566 Da
细胞定位	Antigen Cellular Localization: Mitochondrion
宿主	Goat
种属反应性	Human
亚型	IgG
通用名	MRPS29
基因ID	7818
UniProt ID	P51398
功能	Involved in mediating interferon-gamma-induced cell death.
总结	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q.
形式	0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, ICC, E
别名	28S ribosomal protein S29, mitochondrial, MRP-S29, S29mt, Death-associated protein 3, DAP-3, Ionizing radiation resistance conferring protein, DAP3, MRPS29
图像	AMM04953G (0.5ug/ml) staining of guanidinium thiocyanate-treated HeLa before (left) and after (right) si-RNA-mediated DAP3 knock-down expresson. Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain. AMM04953G (1 μg/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) (35 μg protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence. AMM04953G staining (3 μg/ml) of HeLa lysate (RIPA buffer, 30 μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
说明书	Product Datasheet Protocol
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