您好,欢迎光临安诺伦(北京)生物科技商城!
全国服务热线:400-965-8633
关注我们
  • 会员中心
  • 会员中心
    询价列表
    0

    最新加入的商品

    0 件商品 合计:¥ 0
  • 会员中心
    购物车

    最新加入的商品

    件商品 合计:¥ 0

全部产品分类

自主品牌
当前位置:首页 > 抗原抗体、ELISA、WB > 一抗 > Polyclonal Antibodies > GPX1 Antibody (C-term)

GPX1 Antibody (C-term)

货号 货期 规格 / 价格 询价
AMM05211G 100 μl / ¥4950

GPX1 Antibody (C-term)

品牌

Leading Biology

货号

AMM05211G

产品分类

Polyclonal Antibodies

研究领域

产品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GPX1 antibody (C-term).

分子量

22088 Da

细胞定位

Antigen Cellular Localization: Cytoplasm.

宿主

Rabbit

种属反应性

Human, Mouse, Rat

免疫原

164-193 aa

靶点

This GPX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-193 amino acids from the C-terminal region of human GPX1.

亚型

Rabbit Ig

基因ID

UniProt ID

功能

Protects the hemoglobin in erythrocytes from oxidative breakdown.

总结

GPX1 encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence.

形式

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

储存条件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

应用

FC, WB, IHC-P, IF, E

稀释方法

FC~~1:25 WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50

别名

Glutathione peroxidase 1, GPx-1, GSHPx-1, Cellular glutathione peroxidase, GPX1

图像

Overlay histogram showing Hela cells stained with AMM05211G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM05211G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

All lanes : Anti-GPX1 Antibody (C-term) at 1:2000 dilution Lane 1: rat liver lysate Lane 2: THP-1 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-GPX1 Antibody (C-term) at 1:2000 dilution Lane 1: THP-1 whole cell lysate Lane 2: 293T/17 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: SH-SY5Y whole cell lysate Lane 5: human kidney lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

说明书

数量

相关产品

选择 品牌 货号 产品名称 规格 分类 研究领域 说明书 数量 目录价
1 Leading Biology APR05963G EGFR Antibody (Y1092) 100 μl Polyclonal Antibodies
¥4950.00 订购 询价
2 Leading Biology APR03116G BRCA1 Antibody (aa1847-1863) 100 μl Polyclonal Antibodies
¥4950.00 订购 询价
3 Leading Biology AMM05633G Bombesin Receptor 2 (extracellular) Antibody 50 μl Polyclonal Antibodies
¥6950.00 订购 询价
4 Leading Biology APR10986G AP3B1 Antibody (aa2-14) 50 μg Polyclonal Antibodies
¥4950.00 订购 询价
5 Leading Biology APR11512G Bestrophin-1 (extracellular) Antibody 50 μl Polyclonal Antibodies
¥6950.00 订购 询价
6 Leading Biology APG01407G A3 Adenosine Receptor Antibody 50 μl Polyclonal Antibodies
¥6950.00 订购 询价

产品添加成功!

购物车已有 0 件产品

加入询价单成功!

询价单已有 0 件产品