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Transglutaminase (TGM2) Antibody (Center K444)

APR10532G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR10532G		100 μl / ￥4950

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Transglutaminase (TGM2) Antibody (Center K444)

品牌	Leading Biology	货号	APR10532G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Transglutaminase (TGM2) antibody (Center K444).
分子量	77329 Da
宿主	Rabbit
种属反应性	Human
免疫原	429-458 aa
靶点	This Transglutaminase (TGM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-458 amino acids from the Central region of human Transglutaminase (TGM2).
亚型	Rabbit Ig
基因ID	7052
UniProt ID	P21980
功能	Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
总结	Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. TGM2 acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. It has been identified as the autoantigen implicated in celiac disease.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, IHC-P, IF, E
稀释方法	WB~~1:1000 IF~~1:10~50 IHC-P~~1:10~50
别名	Protein-glutamine gamma-glutamyltransferase 2, Tissue transglutaminase, Transglutaminase C, TG(C), TGC, TGase C, Transglutaminase H, TGase H, Transglutaminase-2, TGase-2, TGM2
图像	Anti-TGM2 Antibody (Center K444) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 77 kDa Blocking/Dilution buffer: 5% NFDM/TBST. TGM2 Antibody (Center K444) (Cat. APR10532G) western blot analysis in HUVEC,K562 cell line lysates (35ug/lane).This demonstrates the TGM2 antibody detected the TGM2 protein (arrow). Fluorescent confocal image of U251 cell stained with TGM2 Antibody (Center K444)( APR10532G).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TGM2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min).TGM2 immunoreactivity is localized to Cytoplasm significantly.
说明书	Product Datasheet Protocol
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