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IRS2 Antibody (C-term)

APR08046G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR08046G		100 μl / ￥4950

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IRS2 Antibody (C-term)

品牌	Leading Biology	货号	APR08046G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality IRS2 antibody (C-term).
分子量	137334 Da
细胞定位	Antigen Cellular Localization: Cytoplasm, cytosol.
宿主	Rabbit
种属反应性	Human
免疫原	1309-1338 aa
靶点	This IRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1309-1338 amino acids from the C-terminal region of human IRS2.
亚型	Rabbit Ig
基因ID	8660
UniProt ID	Q9Y4H2
功能	May mediate the control of various cellular processes by insulin.
总结	Insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. This protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, IHC-P, IF, E
稀释方法	WB~~1:1000 IF~~1:10~50 IHC-P~~1:50~100
别名	Insulin receptor substrate 2, IRS-2, IRS2
图像	Anti-IRS2 Antibody (C-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 137 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Fluorescent image of Hela cell stained with IRS2 Antibody (C-term)( APR08046G).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IRS2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).IRS2 immunoreactivity is localized to Cytoplasm significantly. Western blot analysis of anti-IRS2 Antibody (C-term) (Cat. APR08046G) in 293 cell line lysates (35ug/lane). IRS2 (arrow) was detected using the purified Pab.
说明书	Product Datasheet Protocol
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