品牌 |
Leading Biology | 货号 |
AMR09866G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality SEPT4 antibody (N-term).
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分子量 |
55098 Da
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细胞定位 |
Antigen Cellular Localization:
Cytoplasm. Cytoplasm, cytoskeleton. Note=In platelets, found in areas surrounding alpha-granules.
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宿主 |
Rabbit
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种属反应性 |
Human
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免疫原 |
1-30 aa
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靶点 |
This SEPT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SEPT4.
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亚型 |
Rabbit Ig
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通用名 |
ARTS, PNUTL2, SEP4
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基因ID |
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UniProt ID |
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功能 |
Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion. Isoform ARTS, but not the other isoforms, is required for the induction of cell death mediated by TGF-beta and by other apoptotic stimuli.
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总结 |
PNUTL2 is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. The protein is thought to be part of a complex involved in cytokinesis.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IHC-P, FC, E
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稀释方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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别名 |
Septin-4, Apoptosis-related protein in the TGF-beta signaling pathway, ARTS, Bradeion beta, Brain protein H5, CE5B3 beta, Cell division control-related protein 2, hCDCREL-2, Cerebral protein 7, Peanut-like protein 2, SEPT4, ARTS, PNUTL2, SEP4
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图像 |
Western blot analysis of lysates from SK-N-MC, U-251 MG cell line (from left to right), using PNUTL2 Antibody (N-term)( AMR09866G). AMR09866G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Formalin-fixed and paraffin-embedded human brain tissue reacted with PNUTL2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PNUTL2 Antibody (N-term) (Cat. AMR09866G) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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说明书 |
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数量 |
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