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EDA Antibody (N-term)

AMM06999G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
AMM06999G		100 μl / ￥4950

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EDA Antibody (N-term)

品牌	Leading Biology	货号	AMM06999G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality EDA antibody (N-term).
分子量	41294 Da
细胞定位	Antigen Cellular Localization: Cell membrane; Single-pass type II membrane protein
宿主	Rabbit
种属反应性	Human
免疫原	47-76 aa
靶点	This EDA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-76 amino acids from the N-terminal region of human EDA.
亚型	Rabbit Ig
通用名	ED1, EDA2
基因ID	1896
UniProt ID	Q92838
功能	Seems to be involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Isoform 1 binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor XEDAR.
总结	EDA is a type II membrane protein that can be cleaved by furin to produce a secreted form. This protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in the gene for EDA are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, IHC-P, FC, E
稀释方法	WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50
别名	Ectodysplasin-A, Ectodermal dysplasia protein, EDA protein, Ectodysplasin-A, membrane form, Ectodysplasin-A, secreted form, EDA, ED1, EDA2
图像	Western blot analysis of lysate from RD cell line,using EDA Antibody (N-term)(Cat. AMM06999G).AMM06999G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane. Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EDA antibody (N-term) (Cat. AMM06999G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Flow cytometric analysis of hela cells using EDA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
说明书	Product Datasheet Protocol
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