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当前位置:首页 > 抗原抗体、ELISA、WB > 一抗 > Polyclonal Antibodies > SLC10A1 Antibody (C-term)

SLC10A1 Antibody (C-term)

货号 货期 规格 / 价格 询价
APR09991G 100 μl / ¥4950

SLC10A1 Antibody (C-term)

品牌

Leading Biology

货号

APR09991G

产品分类

Polyclonal Antibodies

研究领域

产品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SLC10A1 antibody (C-term).

分子量

38119 Da

细胞定位

Antigen Cellular Localization: Membrane; Multi-pass membrane protein.

宿主

Rabbit

种属反应性

Human

免疫原

316-343 aa

靶点

This SLC10A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-343 amino acids from the C-terminal region of human SLC10A1.

亚型

Rabbit Ig

通用名

NTCP

基因ID

UniProt ID

功能

The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium.

总结

Sodium/bile acid cotransporters are integral membraneglycoproteins that participate in the enterohepatic circulation ofbile acids. Two homologous transporters are involved in thereabsorption of bile acids, one absorbing from the intestinallumen, the bile duct, and the kidney with an apical localization(SLC10A2; MIM 601295), and the other being found in the basolateralmembranes of hepatocytes (SLC10A1).

形式

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

储存条件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

应用

WB, FC, E

稀释方法

FC~~1:25 WB~~1:2000

别名

Sodium/bile acid cotransporter, Cell growth-inhibiting gene 29 protein, Na(+)/bile acid cotransporter, Na(+)/taurocholate transport protein, Sodium/taurocholate cotransporting polypeptide, Solute carrier family 10 member 1, SLC10A1, NTCP

图像

Overlay histogram showing K562 cells stained with APR09991G (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR09991G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

All lanes : Anti-SLC10A1 Antibody (C-term) at 1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-SLC10A1 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

说明书

数量

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