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当前位置:首页 > 抗原抗体、ELISA、WB > 一抗 > Polyclonal Antibodies > ATP5I Antibody (C-term)

ATP5I Antibody (C-term)

货号 货期 规格 / 价格 询价
APG02134G 100 μl / ¥4950

ATP5I Antibody (C-term)

品牌

Leading Biology

货号

APG02134G

产品分类

Polyclonal Antibodies

研究领域

产品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ATP5I antibody (C-term).

分子量

7933 Da

细胞定位

Antigen Cellular Localization: Mitochondrion. Mitochondrion inner membrane.

宿主

Rabbit

种属反应性

Human, Mouse

靶点

This ATP5I antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 55-89 amino acids from the C-terminal region of human ATP5I.

亚型

Rabbit Ig

通用名

ATP5K

基因ID

UniProt ID

功能

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.

总结

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.

形式

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

储存条件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

应用

WB, IHC-P, IF, E

稀释方法

IF~~1:25 IHC-P~~1:25 WB~~1:1000

别名

ATP synthase subunit e, mitochondrial, ATPase subunit e, ATP5I, ATP5K

图像

Fluorescent image of Hela cells stained with ATP5I Antibody (C-term)( APG02134G). APG02134G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H. liver section using ATP5I Antibody (C-term)( APG02134G). APG02134G was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Fluorescent image of Hela cells stained with ATP5I Antibody (C-term)( APG02134G). APG02134G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).

说明书

数量

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