品牌 |
Leading Biology | 货号 |
APR15604G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CRTAP antibody (N-term).
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分子量 |
46562 Da
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细胞定位 |
Antigen Cellular Localization:
Secreted, extracellular space, extracellular matrix
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宿主 |
Rabbit
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种属反应性 |
Human
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免疫原 |
31-59 aa
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靶点 |
This CRTAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-59 amino acids from the N-terminal region of human CRTAP.
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亚型 |
Rabbit Ig
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通用名 |
CASP
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基因ID |
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UniProt ID |
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功能 |
Necessary for efficient 3-hydroxylation of fibrillar collagen prolyl residues.
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总结 |
The protein encoded by this gene is similar to the chickenand mouse CRTAP genes. The encoded protein is a scaffolding proteinthat may influence the activity of at least one member of thecytohesin/ARNO family in response to specific cellular stimuli.Defects in this gene are associated with osteogenesis imperfecta, aconnective tissue disorder characterized by bone fragility and lowbone mass.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IF, E
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稀释方法 |
IF~~1:25
WB~~1:1000
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别名 |
Cartilage-associated protein, CRTAP, CASP
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图像 |
Fluorescent image of Hela cells stained with CRTAP Antibody (N-term)( APR15604G). APR15604G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).
Western blot analysis of lysates from HT-1080, ZR-75-1 cell line (from left to right), using CRTAP Antibody (N-term)( APR15604G). APR15604G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
CRTAP Antibody (N-term) (Cat. APR15604G) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CRTAP antibody detected the CRTAP protein (arrow). |
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说明书 |
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数量 |
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