> 抗原抗体、ELISA、WB > 一抗 > Polyclonal Antibodies > Alkaline Phosphatase (ALPI) Antibody (Center)品牌 |
Leading Biology | 货号 |
APR07058G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Alkaline Phosphatase (ALPI) antibody (Center).
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分子量 |
56812 Da
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细胞定位 |
Antigen Cellular Localization:
Cell membrane; Lipid-anchor, GPI-anchor.
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宿主 |
Rabbit
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种属反应性 |
Human
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免疫原 |
274-304 aa
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靶点 |
This Alkaline Phosphatase (ALPI) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-304 amino acids from the Central region of human Alkaline Phosphatase (ALPI).
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亚型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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总结 |
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
IF, WB, IHC-P, E
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稀释方法 |
WB~~1:1000
IHC-P~~1:100
IF~~1:100
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别名 |
Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, ALPI
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图像 |
Western blot analysis of lysate from human placenta tissue lysate, using ALPI Antibody (Center)( APR07058G). APR07058G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Immunohistochemical analysis of paraffin-embedded H. small intestine section using Alkaline Phosphatase (ALPI) Antibody(Center) ( APR07058G). APR07058G was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysate from human placenta tissue,using ALPI Antibody (Center)(Cat. APR07058G).APR07058G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane. |
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说明书 |
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数量 |
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