品牌 |
Leading Biology | 货号 |
AMM05746G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CHAT antibody (N-term).
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分子量 |
82536 Da
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宿主 |
Rabbit
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种属反应性 |
Human, Mouse
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免疫原 |
98-128 aa
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靶点 |
This CHAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-128 amino acids from the N-terminal region of human CHAT.
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亚型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
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总结 |
CHAT is an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IF, E
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稀释方法 |
WB~~1:1000
IF~~1:10~50
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别名 |
Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, CHAT
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图像 |
Western blot analysis of CHAT Antibody (N-term) (Cat. AMM05746G) in NCI-H460 cell line lysates (35ug/lane).CHAT (arrow) was detected using the purified Pab.
Western blot analysis of CHAT Antibody (N-term) (Cat. AMM05746G) in mouse liver tissue lysates (35ug/lane). CHAT (arrow) was detected using the purified Pab.
Confocal immunofluorescent analysis of CHAT Antibody (N-term) (Cat. AMM05746G) with NCI-H460 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
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说明书 |
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数量 |
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