品牌 |
Leading Biology | 货号 |
APR16947G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ITPKA antibody (C-term).
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分子量 |
51009 Da
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宿主 |
Rabbit
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种属反应性 |
Human, Mouse
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免疫原 |
345-375 aa
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靶点 |
This ITPKA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 345-375 amino acids from the C-terminal region of human ITPKA.
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亚型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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总结 |
ITPKA regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of the inositol 1,4,5-trisphosphate 3-kinase is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. It is also a substrate for the cyclic AMP-dependent protein kinase, calcium/calmodulin- dependent protein kinase II, and protein kinase C in vitro. ITPKA and ITPKB are 68% identical in the C-terminus region.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, E
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稀释方法 |
WB~~1:1000
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别名 |
Inositol-trisphosphate 3-kinase A, Inositol 1, 5-trisphosphate 3-kinase A, IP3 3-kinase A, IP3K A, InsP 3-kinase A, ITPKA
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图像 |
All lanes : Anti-ITPKA Antibody (C-term) at 1:1000 dilution Lane 1: human brain lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of hITPKA-E360 (Cat. APR16947G) in K562 cell line and mouse lung tissue lysates (35ug/lane). ITPKA (arrow) was detected using the purified Pab. |
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说明书 |
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数量 |
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