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当前位置:首页 > 抗原抗体、ELISA、WB > 一抗 > Polyclonal Antibodies > AK3 Antibody (C-term H38)

AK3 Antibody (C-term H38)

货号 货期 规格 / 价格 询价
APG01642G 100 μl / ¥4950

AK3 Antibody (C-term H38)

品牌

Leading Biology

货号

APG01642G

产品分类

Polyclonal Antibodies

研究领域

产品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality AK3 antibody (C-term H38).

分子量

25268 Da

细胞定位

Antigen Cellular Localization: Mitochondrion matrix {ECO:0000255|HAMAP- Rule:MF_03170, ECO:0000269|PubMed:11485571, ECO:0000269|PubMed:19766732}

宿主

Rabbit

种属反应性

Human, Mouse

免疫原

195-223 aa

靶点

This AK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-223 amino acids from the C-terminal region of human AK3.

亚型

Rabbit Ig

基因ID

UniProt ID

功能

Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity.

总结

AK3 is a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated.

形式

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

储存条件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

应用

WB, IHC-P, E

稀释方法

WB~~1:1000 IHC-P~~1:50~100

别名

Adenylate kinase 4, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03170}, AK 4 {ECO:0000255|HAMAP-Rule:MF_03170}, 27410 {ECO:0000255|HAMAP-Rule:MF_03170}, 2746 {ECO:0000255|HAMAP-Rule:MF_03170}, Adenylate kinase 3-like {ECO:0000255|HAMAP-Rule:MF_03170}, GTP:AMP phosphotransferase AK4 {ECO:0000255|HAMAP-Rule:MF_03170}, AK4 {ECO:0000255|HAMAP-Rule:MF_03170}

图像

The anti-AK3 Pab (Cat. APG01642G) is used in Western blot to detect AK3 in mouse kidney tissue lysate.

Western blot analysis of AK3 (arrow) using rabbit polyclonal AK3 Antibody (C-term H38) (Cat. APG01642G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AK3 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

说明书

数量

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