品牌 |
Leading Biology | 货号 |
APR07100G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality DGKB antibody (C-term).
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分子量 |
90595 Da
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细胞定位 |
Antigen Cellular Localization:
Cytoplasm.
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宿主 |
Rabbit
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种属反应性 |
Human, Mouse
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免疫原 |
773-804 aa
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靶点 |
This DGKB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 773-804 amino acids from the C-terminal region of human DGKB.
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亚型 |
Rabbit Ig
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通用名 |
DAGK2, KIAA0718
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基因ID |
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UniProt ID |
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功能 |
Exhibits high phosphorylation activity for long-chain diacylglycerols.
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总结 |
Diacylglycerol (DAG) is an allosteric activator of protein kinase C. DAG also participates in regulating RAS and RHO family proteins by activating the guanine nucleotide exchange factors VAV and RASGRP1. DAG is also involved in the synthesis of phospholipids and triacylglycerols. Tight regulation of DAG levels is achieved via DAG kinases (DGKs), which remove DAG by phosphorylate it to phosphatidic acid. Several mammalian isozymes of DAGK have been identified
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IHC-P, E
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稀释方法 |
WB~~1:1000
IHC-P~~1:50~100
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别名 |
Diacylglycerol kinase beta, DAG kinase beta, 90 kDa diacylglycerol kinase, Diglyceride kinase beta, DGK-beta, DGKB, DAGK2, KIAA0718
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图像 |
Western blot analysis of anti-hDGKB-T787 Pab (Cat. APR07100G) in mouse liver tissue lysate. hDGKB-T787(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
DGKB Antibody (T787) (Cat. APR07100G) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the DGKB antibody detected the DGKB protein (arrow). |
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说明书 |
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数量 |
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