全部产品分类
品牌 |
Leading Biology | 货号 |
AMM07183G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PI4K2A (PI4K II) antibody (N-term).
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分子量 |
54022 Da
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细胞定位 |
Antigen Cellular Localization:
Golgi apparatus, trans-Golgi network membrane; Lipid- anchor. Membrane raft. Cell projection, dendrite {ECO:0000250|UniProtKB:Q2TBE6}. Cell junction, synapse, presynaptic cell membrane {ECO:0000250|UniProtKB:Q2TBE6}. Cell junction, synapse, synaptosome {ECO:0000250|UniProtKB:Q2TBE6} Mitochondrion {ECO:0000250|UniProtKB:Q2TBE6}. Endosome Cytoplasmic vesicle. Membrane; Lipid-anchor. Cell membrane Note=Localized in neuronal cell body. Transported from neuronal cell body to neuron projections in a BLOC-1- and AP-3-complexes- dependent manner. Enriched in neurite tips and neuron projections in a BLOC-1- and AP-3-complexes-dependent manner (By similarity) Found in subdomains of the plasma membrane termed non-caveolar membrane rafts. {ECO:0000250|UniProtKB:Q2TBE6, ECO:0000250|UniProtKB:Q99M64}
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宿主 |
Rabbit
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种属反应性 |
Human
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免疫原 |
1-30 aa
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靶点 |
This PI4K2A (PI4K II) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PI4K2A (PI4K II).
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亚型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Membrane-bound phosphatidylinositol-4 kinase (PI4- kinase) that catalyzes the phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P), a lipid that plays important roles in endocytosis, Golgi function, protein sorting and membrane trafficking and is required for prolonged survival of neurons. Besides, phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P) is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3).
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总结 |
Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs. PI4K II is activated by detergent and inhibited by adenosine. Overexpression of PI4KII in COS-7 cells increases synthesis of PtdIns4P. Some cells overexpressing PI4KII have scattered or no perinuclear Golgi. Knockdown of PI4KII by RNA interference (RNAi) does not disrupt the Golgi, and some cells show expanded Golgi. RNAi reduces the Golgi level of PtdIns4P and blocks the association between AP1 and the trans-Golgi network. PI4KII RNAi had little effect on intra-Golgi trafficking, but it inhibited export to plasma membrane export by 35%. It has been proposed that PI4KII generates PtdIns4P-rich domains within the Golgi that specify docking of the AP1 coat machinery.
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形式 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IHC-P, IF, E
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稀释方法 |
WB~~1:1000
IHC-P~~1:50~100
IF~~1:10~50
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别名 |
Phosphatidylinositol 4-kinase type 2-alpha, Phosphatidylinositol 4-kinase type II-alpha, PI4K2A
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图像 |
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说明书 |
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数量 |
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