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当前位置:首页 > 抗原抗体、ELISA、WB > 一抗 > Polyclonal Antibodies > GCAT Antibody (Center)

GCAT Antibody (Center)

货号 货期 规格 / 价格 询价
AMM04727G 100 μl / ¥4950

GCAT Antibody (Center)

品牌

Leading Biology

货号

AMM04727G

产品分类

Polyclonal Antibodies

研究领域

产品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GCAT antibody (Center).

分子量

45285 Da

细胞定位

Antigen Cellular Localization: Mitochondrion. Nucleus. Note=Translocates to the nucleus upon cold and osmotic stress

宿主

Rabbit

种属反应性

Human, Mouse

免疫原

155-181 aa

靶点

This GCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-181 amino acids from the Central region of human GCAT.

亚型

Rabbit Ig

通用名

KBL

基因ID

UniProt ID

总结

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase.

形式

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

储存条件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

应用

WB, IHC-P, FC, E

稀释方法

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

别名

2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, AKB ligase, Aminoacetone synthase, Glycine acetyltransferase, GCAT, KBL

图像

Western blot analysis of GCAT antibody (Center) (Cat. AMM04727G) in mouse liver tissue lysates (35ug/lane). GCAT (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain reacted with GCAT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of HepG2 cells using GCAT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

说明书

数量

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