品牌 |
Leading Biology | 货号 |
AMM05657G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CA14 antibody (N-term).
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分子量 |
37668 Da
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细胞定位 |
Antigen Cellular Localization:
Membrane; Single-pass type I membrane protein
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宿主 |
Rabbit
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种属反应性 |
Human
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免疫原 |
51-80 aa
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靶点 |
This CA14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-80 amino acids from the N-terminal region of human CA14.
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亚型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Reversible hydration of carbon dioxide.
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总结 |
CA14 belongs a large family of zinc metalloenzymes that catalyze the versible hydration of carbon dioxide. The protein is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, FC, E
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稀释方法 |
WB~~1:1000
FC~~1:10~50
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别名 |
Carbonic anhydrase 14, Carbonate dehydratase XIV, Carbonic anhydrase XIV, CA-XIV, CA14
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图像 |
Western blot analysis of CA14 antibody (N-term) (Cat. AMM05657G) in A375 cell line lysates (35ug/lane). CA14 (arrow) was detected using the purified Pab.
Flow cytometric analysis of A375 cells using CA14 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CA14 (arrow) using rabbit polyclonal CA14 Antibody (N-term) (Cat. AMM05657G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA14 gene. |
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说明书 |
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数量 |
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