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当前位置:首页 > 抗原抗体、ELISA、WB > 一抗 > Polyclonal Antibodies > CKM Antibody (C-term)

CKM Antibody (C-term)

货号 货期 规格 / 价格 询价
APR07089G 100 μl / ¥4950

CKM Antibody (C-term)

品牌

Leading Biology

货号

APR07089G

产品分类

Polyclonal Antibodies

研究领域

产品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CKM antibody (C-term).

分子量

43101 Da

细胞定位

Antigen Cellular Localization: Cytoplasm.

宿主

Rabbit

种属反应性

Human

免疫原

283-313 aa

靶点

This CKM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-313 amino acids from the C-terminal region of human CKM.

亚型

Rabbit Ig

通用名

CKMM

基因ID

UniProt ID

功能

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

总结

Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. The CKM isoform, predominant in skeletal muscle and heart tissue, is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

形式

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

储存条件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

应用

WB, IHC-P, FC, E

稀释方法

WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50

别名

Creatine kinase M-type, Creatine kinase M chain, M-CK, Creatine kinase M-type, N-terminally processed, CKM, CKMM

图像

The anti-CKM Pab (Cat. APR07089G) is used in Western blot to detect CKM in C6 cell lysate.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Flow cytometric analysis of HepG2 cells using Creatine Kinase MB (CKM) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

说明书

数量

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