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AQP1 Antibody

APG01959G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APG01959G		100 μl / ￥4950

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安诺伦（北京）生物科技有限公司

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AQP1 Antibody

品牌	Leading Biology	货号	APG01959G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality AQP1 antibody.
分子量	28; [uniprot]28,20,23,17; KDa
细胞定位	Antigen Cellular Localization: Cell membrane; Multi-pass membrane protein
宿主	Rabbit
种属反应性	Human, Mouse, Rat, Dog, Sheep
免疫原	181-269/269 aa
通用名	CHIP28
基因ID	358
UniProt ID	P29972
功能	Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
总结	Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
形式	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB
稀释方法	WB~~1:100~1:500 IHC-P~~1:100~1:500
别名	Aquaporin-1, AQP-1, Aquaporin-CHIP, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule, AQP1, CHIP28
图像	Lane 1: mouse heart lysates Lane 2: mouse lung lysates probed with Anti AQP1/CHIP Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 in 4?C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37?C. Predicted band 28kD. Observed band size: 28kD. Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AQP1 Polyclonal Antibody, Unconjugated (APG01959G) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AQP1 Polyclonal Antibody, Unconjugated (bs-1506R) at 1:200 overnight at 4°C, followed by a conjugated secondary (bs-0295G-AF488) at [1:500] for 90 minutes and DAPI staining of the nuclei.
说明书	Product Datasheet Protocol
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