品牌 |
Leading Biology | 货号 |
APR09161G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Phospho-eNos(S1177) antibody.
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分子量 |
133289 Da
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细胞定位 |
Antigen Cellular Localization:
Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity
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宿主 |
Rabbit
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种属反应性 |
Human
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靶点 |
This eNos Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1177 of human eNos.
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亚型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
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总结 |
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
DB, IF, E
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稀释方法 |
DB~~1:500
IF~~1:10~50
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别名 |
Nitric oxide synthase, endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, eNOS, NOS type III, NOSIII, NOS3
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图像 |
Dot blot analysis of anti-Phospho-eNos-S1177 Phospho-specific Pab (Cat. APR09161G) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Confocal immunofluorescent analysis of Phospho-eNos-S1177 Antibody ( APR09161G) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
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说明书 |
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数量 |
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