品牌 |
Leading Biology | 货号 |
AMM04459G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality ETFA antibody (Center).
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分子量 |
35080 Da
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细胞定位 |
Antigen Cellular Localization:
Mitochondrion matrix.
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宿主 |
Rabbit
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种属反应性 |
Human, Mouse
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靶点 |
This ETFA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 192-226 amino acids from the Central region of human ETFA.
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亚型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
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总结 |
The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IF, E
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稀释方法 |
IF~~1:25
WB~~1:1000
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别名 |
Electron transfer flavoprotein subunit alpha, mitochondrial, Alpha-ETF, ETFA
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图像 |
Fluorescent image of Hela cells stained with ETFA Antibody (Center)( AMM04459G). AMM04459G was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).
Western blot analysis of lysates from A431, Hela, HepG2 cell line , human liver and mouse liver tissue lysate(from left to right), using ETFA Antibody (Center)( AMM04459G). AMM04459G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. |
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说明书 |
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数量 |
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