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当前位置:首页 > 抗原抗体、ELISA、WB > 一抗 > Polyclonal Antibodies > GSTT1 Antibody (N-term)

GSTT1 Antibody (N-term)

货号 货期 规格 / 价格 询价
AMM05271G 100 μl / ¥4950

GSTT1 Antibody (N-term)

品牌

Leading Biology

货号

AMM05271G

产品分类

Polyclonal Antibodies

研究领域

产品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality GSTT1 antibody (N-term).

分子量

27335 Da

细胞定位

Antigen Cellular Localization: Cytoplasm.

宿主

Rabbit

种属反应性

Human

免疫原

7-34 aa

靶点

This GSTT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-34 amino acids from the N-terminal region of human GSTT1.

亚型

Rabbit Ig

基因ID

UniProt ID

功能

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.

总结

Glutathione S-transferase (GST) theta 1 (GSTT1) is amember of a superfamily of proteins that catalyze the conjugationof reduced glutathione to a variety of electrophilic andhydrophobic compounds. Human GSTs can be divided into five mainclasses: alpha, mu, pi, theta, and zeta. The theta class includesGSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequenceidentity and both of them were claimed to have an important role inhuman carcinogenesis. The GSTT1 gene is located approximately 50kbaway from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similarstructure, being composed of five exons with identical exon/intronboundaries.

形式

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

储存条件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

应用

WB, FC, E

稀释方法

WB~~1:1000 FC~~1:10~50

别名

Glutathione S-transferase theta-1, GST class-theta-1, Glutathione transferase T1-1, GSTT1

图像

Anti-GSTT1 Antibody (N-term)at 1:2000 dilution + human lung lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of GSTT1 (arrow) using rabbit polyclonal GSTT1 Antibody (N-term) (Cat. AMM05271G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GSTT1 gene.

GSTT1 Antibody (N-term) (Cat. AMM05271G) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

说明书

数量

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