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KRT12 Antibody (C-term)

AMM06212G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
AMM06212G		100 μl / ￥4950

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KRT12 Antibody (C-term)

品牌	Leading Biology	货号	AMM06212G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality KRT12 antibody (C-term).
分子量	53511 Da
宿主	Rabbit
种属反应性	Human, Mouse, Rat
免疫原	442-471 aa
靶点	This KRT12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-471 amino acids from the C-terminal region of human KRT12.
亚型	Rabbit Ig
基因ID	3859
UniProt ID	Q99456
功能	May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity (By similarity).
总结	KRT12 encodes the type I intermediate filament chainkeratin 12, expressed in corneal epithelia. Mutations in this genelead to Meesmann corneal dystrophy.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, IHC-P, FC, E
稀释方法	FC~~1:25 WB~~1:1000 IHC-P~~1:10~50
别名	Keratin, type I cytoskeletal 12, Cytokeratin-12, CK-12, Keratin-12, K12, KRT12
图像	Overlay histogram showing K562 cells stained with AMM06212G (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM06212G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Anti-KRT12 Antibody (C-term) at 1:2000 dilution + rat eyeball lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Anti-KRT12 Antibody (C-term)at 1:2000 dilution + mouse eyeball lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
说明书	Product Datasheet Protocol
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