品牌 |
Leading Biology | 货号 |
APR15480G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CLDN2 antibody (C-term Y224).
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分子量 |
24549 Da
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细胞定位 |
Antigen Cellular Localization:
Cell junction, tight junction. Cell membrane; Multi-pass membrane protein
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宿主 |
Rabbit
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种属反应性 |
Human
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免疫原 |
200-227 aa
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靶点 |
This CLDN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-227 amino acids from the C-terminal region of human CLDN2.
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亚型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity.
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总结 |
This gene product belongs to the claudin protein familywhose members have been identified as major integral membraneproteins localized exclusively at tight junctions. Claudins areexpressed in an organ-specific manner and regulate tissue-specificphysiologic properties of tight junctions. This protein isexpressed in the intestine. Alternatively spliced transcriptvariants with different 5' untranslated region have been found forthis gene.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IHC-P, FC, E
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稀释方法 |
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
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别名 |
Claudin-2, SP82, CLDN2
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图像 |
Anti-CLDN2 Antibody at 1:1000 dilution + human heart lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of CLDN2 (arrow) using rabbit polyclonal CLDN2 Antibody (Y224) (Cat. APR15480G). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLDN2 gene.
CLDN2 Antibody (C-term Y224) (Cat. APR15480G)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CLDN2 Antibody (C-term Y224) for immunohistochemistry. Clinical relevance has not been evaluated. |
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说明书 |
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数量 |
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