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SDHD Antibody (N-term)

AMR09852G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
AMR09852G		100 μl / ￥4950

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安诺伦（北京）生物科技有限公司

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SDHD Antibody (N-term)

品牌	Leading Biology	货号	AMR09852G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality SDHD antibody (N-term).
分子量	17043 Da
细胞定位	Antigen Cellular Localization: Mitochondrion inner membrane; Multi-pass membrane protein
宿主	Rabbit
种属反应性	Human
免疫原	13-42 aa
靶点	This SDHD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human SDHD.
亚型	Rabbit Ig
通用名	SDH4
基因ID	6392
UniProt ID	O14521
功能	Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
总结	Complex II of the respiratory chain, which isspecifically involved in the oxidation of succinate, carrieselectrons from FADH to CoQ. The complex is composed of fournuclear-encoded subunits and is localized in the mitochondrialinner membrane. The subunit D protein is one of two integralmembrane proteins anchoring the complex to the matrix side of themembrane. Mutations in SDHD have been linked to hereditaryparaganglioma.
形式	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, IHC-P, IF, FC, E
稀释方法	WB~~1:1000 IHC-P~~1:10~50 IF~~1:10~50 FC~~1:10~50
别名	Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial, CybS, CII-4, QPs3, Succinate dehydrogenase complex subunit D, Succinate-ubiquinone oxidoreductase cytochrome b small subunit, Succinate-ubiquinone reductase membrane anchor subunit, SDHD, SDH4
图像	All lanes : Anti-SDHD Antibody (N-term) at 1:2000 dilution Lane 1: A2058 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST. SDHD Antibody (N-term) (Cat. AMR09852G) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SDHD antibody detected the SDHD protein (arrow). SDHD Antibody (N-term) (Cat. AMR09852G)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SDHD Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
说明书	Product Datasheet Protocol
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