品牌 |
Leading Biology | 货号 |
AMM07041G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PDE1B antibody (C-term).
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分子量 |
61380 Da
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细胞定位 |
Antigen Cellular Localization:
Cytoplasm.
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宿主 |
Rabbit
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种属反应性 |
Human, Mouse
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免疫原 |
473-500 aa
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靶点 |
This PDE1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 473-500 amino acids from the C-terminal region of human PDE1B.
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亚型 |
Rabbit Ig
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通用名 |
PDE1B1, PDES1B
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基因ID |
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UniProt ID |
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功能 |
Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate.
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总结 |
Cyclic nucleotide phosphodiesterases (PDEs) catalyzehydrolysis of the cyclic nucleotides cAMP and cGMP to thecorresponding nucleoside 5-prime-monophosphates. Mammalian PDEshave been classified into several families based on theirbiochemical properties. Members of the PDE1 family, such as PDE1B,are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that arestimulated by a calcium-calmodulin complex (Repaske et al., 1992[PubMed 1326532]).
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IHC-P, E
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稀释方法 |
WB~~1:1000
IHC-P~~1:10~50
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别名 |
Calcium/calmodulin-dependent 3', 5'-cyclic nucleotide phosphodiesterase 1B, Cam-PDE 1B, 63 kDa Cam-PDE, PDE1B, PDE1B1, PDES1B
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图像 |
Anti-PDE1B Antibody (C-term) at 1:1000 dilution + THP-1 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
PDE1B Antibody (C-term) (Cat. AMM07041G) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the PDE1B antibody detected the PDE1B protein (arrow).
PDE1B Antibody (C-term) (Cat. AMM07041G)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PDE1B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
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说明书 |
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数量 |
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