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当前位置:首页 > 抗原抗体、ELISA、WB > 一抗 > Polyclonal Antibodies > HSD17B12 Antibody (Center)

HSD17B12 Antibody (Center)

货号 货期 规格 / 价格 询价
APR10884G 100 μl / ¥5450

HSD17B12 Antibody (Center)

品牌

Leading Biology

货号

APR10884G

产品分类

Polyclonal Antibodies

研究领域

产品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality HSD17B12 antibody (Center).

分子量

34324 Da

细胞定位

Antigen Cellular Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein

宿主

Rabbit

种属反应性

Human

免疫原

126-155 aa

靶点

This HSD17B12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acids from the Central region of human HSD17B12.

亚型

Rabbit Ig

基因ID

UniProt ID

功能

Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation.

总结

The enzyme 17-beta hydroxysteroid dehydrogenase-12 (HSD17B12) uses NADPH to reduce 3-ketoacyl-CoA to 3-hydroxyacyl-CoA during the second step of fatty acid elongation.

形式

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

储存条件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

应用

WB, IHC-P, FC, E

稀释方法

WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50

别名

Estradiol 17-beta-dehydrogenase 12, 17-beta-hydroxysteroid dehydrogenase 12, 17-beta-HSD 12, 3-ketoacyl-CoA reductase, KAR, 131-, HSD17B12

图像

HSD17B12 Antibody (Center) (Cat. APR10884G) western blot analysis in A431,SiHa cell line lysates (35ug/lane).This demonstrates the HSD17B12 antibody detected the HSD17B12 protein (arrow).

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HSD17B12 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

HSD17B12 Antibody (Center) (Cat. APR10884G) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

说明书

数量

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