品牌 |
Leading Biology | 货号 |
AMM08684G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality SIL1 antibody (Center).
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分子量 |
52085 Da
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细胞定位 |
Antigen Cellular Localization:
Endoplasmic reticulum lumen
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宿主 |
Rabbit
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种属反应性 |
Human, Mouse
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免疫原 |
99-126 aa
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靶点 |
This SIL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-126 amino acids from the Central region of human SIL1.
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亚型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.
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总结 |
SIL1 IS a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IHC-P, FC, E
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稀释方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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别名 |
Nucleotide exchange factor SIL1, BiP-associated protein, BAP, SIL1
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图像 |
Western blot analysis of SIL1 Antibody (Center) (Cat. AMM08684G) in mouse kidney tissue lysates (35ug/lane).SIL1 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SIL1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of HepG2 cells using SIL1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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说明书 |
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数量 |
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