品牌 |
Leading Biology | 货号 |
APR03391G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Phospho-Thr210 Polo-like Kinase 1 Antibody.
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分子量 |
66 KDa
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宿主 |
Rabbit
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种属反应性 |
Human, Rat
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靶点 |
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr210 conjugated to KLH.
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基因ID |
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UniProt ID |
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总结 |
Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004). Deregulated expression of PLK’s is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005). It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser137 and Thr210 (van de Weerdt et al., 2005).
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB
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稀释方法 |
WB~~ 1:1000
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图像 |
Western blot of rat synaptic membrane showing specific immunolabeling of the~66 k PLK protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). Theblot is identical to the control except that it was incubated in λ-Ptase (1200 unitsfor 30 min) before being exposed to the phospho-Thr210 PLK antibody. The immunolabeling is completely eliminated by treatment with λ-Ptase. |
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说明书 |
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数量 |
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