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BMI1 Antibody (C-term)

APR06944G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR06944G		100 μl / ￥4950

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BMI1 Antibody (C-term)

品牌	Leading Biology	货号	APR06944G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality BMI1 Antibody (C-term).
分子量	H=37;M=37 KDa
细胞定位	Antigen Cellular Localization: Nucleus. Cytoplasm.
宿主	Rabbit
种属反应性	Human, Mouse
免疫原	281-314 aa
靶点	This BMI1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 281-314 amino acids from the C-terminal region of human BMI1.
通用名	PCGF4, RNF51
基因ID	100532731;648
UniProt ID	P35226
功能	Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
总结	Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	IF, WB
稀释方法	WB~~1:1000 IF~~1:25
图像	All lanes : Anti-BMI1 Antibody (C-term) at 1:1000 dilution Lane 1: A549 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: MCF-7 whole cell lysates Lane 4: NIH/3T3 whole cell lysates Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human cervical epithelial adenocarcinoma cell line) cells labeling BMI1 with APR06944G at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor? 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).
说明书	Product Datasheet Protocol
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