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当前位置:首页 > 抗原抗体、ELISA、WB > 一抗 > Polyclonal Antibodies > PIK3R3 Antibody (C-term)

PIK3R3 Antibody (C-term)

货号 货期 规格 / 价格 询价
APR06052G 100 μl / ¥4950

PIK3R3 Antibody (C-term)

品牌

Leading Biology

货号

APR06052G

产品分类

Polyclonal Antibodies

研究领域

产品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PIK3R3 Antibody (C-term).

分子量

54448 Da

宿主

Rabbit

种属反应性

Human

免疫原

316-346 aa

靶点

This PIK3R3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-346 amino acids from the C-terminal region of human PIK3R3.

亚型

Rabbit Ig

基因ID

UniProt ID

功能

Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.

总结

PIK3R3 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The protein is expressed at highest levels in brain and testis. Lower levels are detected in adipose tissue, kidney, heart, lung and skeletal muscle. The protein contains 2 SH2 domains.

储存条件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

应用

WB, IHC-P, IF, E

稀释方法

IF~~1:100 WB~~1:1000 IHC-P~~1:10~50

图像

Fluorescent image of U251 cells stained with PIK3R3 Antibody(C-term) (APR06052G). APR06052G was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).

Western blot analysis of lysate from MDA-MB-453 cell line, using PI3KR3 Antibody (G331)(Cat. APR06052G. RB1719 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIK3R3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

说明书

数量

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