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Mouse Pbk Antibody(N-term)

APR04430G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR04430G		100 μl / ￥4950

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Mouse Pbk Antibody(N-term)

品牌	Leading Biology	货号	APR04430G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Mouse Pbk Antibody(N-term).
分子量	36745 Da
宿主	Rabbit
种属反应性	Human, Mouse
免疫原	58-86 aa
靶点	This Mouse Pbk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the N-terminal region of mouse Pbk.
亚型	Rabbit Ig
通用名	Topk
基因ID	52033
UniProt ID	Q9JJ78
功能	Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization (By similarity).
总结	Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization (By similarity).
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, IHC-P, FC, E
稀释方法	FC~~1:25 IHC-P~~1:25 WB~~1:1000
图像	Overlay histogram showing A431 cells stained with APR04430G (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR04430G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Immunohistochemical analysis of paraffin-embedded R. testis section using Mouse Pbk Antibody(N-term)( APR04430G). APR04430G was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining. Western blot analysis of lysate from Hela cell line, using Mouse Pbk Antibody (N-term)(APR04430G). APR04430G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
说明书	Product Datasheet Protocol
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