品牌 |
Leading Biology | 货号 |
APR04153G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality PTTG2 Antibody (Center).
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分子量 |
22302 Da
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细胞定位 |
Antigen Cellular Localization:
Cytoplasm. Nucleus
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宿主 |
Rabbit
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种属反应性 |
Human
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免疫原 |
56-84 aa
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靶点 |
This PTTG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-84 amino acids from the Central region of human PTTG2.
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亚型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IF, E
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稀释方法 |
WB~~1:1000
IF~~1:10~50
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图像 |
Anti-PTTG2 Antibody (Center) at 1:2000 dilution + human liver lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
PTTG2 Antibody (Center) (Cat. APR04153G) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PTTG2 antibody detected the PTTG2 protein (arrow).
Fluorescent confocal image of MCF-7 cell stained with PTTG2 Antibody (Center)( APR04153G).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PTTG2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). PTTG2 immunoreactivity is localized to Cytoplasm significantly. |
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说明书 |
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数量 |
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