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PREX1 Antibody (C-term)

APR03713G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR03713G		100 μl / ￥4950

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PREX1 Antibody (C-term)

品牌	Leading Biology	货号	APR03713G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PREX1 Antibody (C-term).
分子量	186203 Da
细胞定位	Antigen Cellular Localization: Cytoplasm, cytosol. Cell membrane. Note=Mainly cytosolic. Some amount is apparently associated to the plasma membrane
宿主	Rabbit
种属反应性	Human
免疫原	1134-1163 aa
靶点	This PREX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1134-1163 amino acids from the C-terminal region of human PREX1.
亚型	Rabbit Ig
通用名	KIAA1415
基因ID	57580
UniProt ID	Q8TCU6
功能	Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils.
总结	The protein encoded by this gene acts as a guaninenucleotide exchange factor for the RHO family of small GTP-bindingproteins (RACs). It has been shown to bind to and activate RAC1 byexchanging bound GDP for free GTP. The encoded protein, which isfound mainly in the cytoplasm, is activated byphosphatidylinositol-3,4,5-trisphosphate and the beta-gammasubunits of heterotrimeric G proteins.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	IHC-P, WB, FC, E
稀释方法	IHC-P~~1:10~50 WB~~1:1000 FC~~1:10~50
图像	APR03713G staining PREX1 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-PREX1 Antibody (C-term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: SK-BR-3 whole cell lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 186 kDa Blocking/Dilution buffer: 5% NFDM/TBST. PREX1 Antibody (C-term) (Cat. APR03713G) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PREX1 antibody detected the PREX1 protein (arrow).
说明书	Product Datasheet Protocol
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