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CF150 Antibody (Center)

APR03444G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR03444G		100 μl / ￥4950

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安诺伦（北京）生物科技有限公司

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CF150 Antibody (Center)

品牌	Leading Biology	货号	APR03444G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CF150 Antibody (Center).
分子量	58814 Da
细胞定位	Antigen Cellular Localization: Cytoplasm, cytosol
宿主	Rabbit
种属反应性	Human
免疫原	266-295 aa
靶点	This CF150 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human CF150.
亚型	Rabbit Ig
通用名	C6orf150
基因ID	115004
UniProt ID	Q8N884
功能	Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double- stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.
总结	The exact function of C6orf150 remains unknown.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, FC, E
稀释方法	FC~~1:10~50 WB~~1:500-1:1000
图像	Overlay histogram showing U-2OS cells stained with APR03444G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR03444G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Flow cytometric analysis of A549 cells using CF150 Antibody (Center) (green, APR03444G) compared to an isotype control of rabbit IgG(blue). APR03444G was diluted at 1:25 dilution. An Alexa Fluor? 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody. Western blot analysis of lysate from THP-1 cell line, using CF150 Antibody (Center)(APR03444G). APR03444G was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
说明书	Product Datasheet Protocol
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