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CD73(NT5E) Antibody (C-term)

AMM02468G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
AMM02468G		100μl / ￥4950

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CD73(NT5E) Antibody (C-term)

品牌	Leading Biology	货号	AMM02468G
产品分类	Monoclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CD73(NT5E) Antibody (C-term).
分子量	63368 Da
细胞定位	Antigen Cellular Localization: Cell membrane; Lipid-anchor, GPI-anchor.
宿主	Mouse
种属反应性	Human
靶点	Purified His-tagged CD73(NT5E) protein was used to produced this monoclonal antibody.
克隆号	974CT7.5.3
亚型	IgG1
通用名	NT5, NTE
基因ID	4907
UniProt ID	P21589
功能	Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
总结	Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	IHC-P, WB, E
稀释方法	IHC-P~~1:25 WB~~1:5000
图像	AM2223A staining CD73 (NT5E) in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. AM2223A staining CD73 (NT5E) in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Anti-CD73 (NT5E) Antibody (C-term) at 1:4000 dilution + MDA-MB-231 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 63 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
说明书	Product Datasheet Protocol
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