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Cyclin E1 Antibody

APR00378G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR00378G		50 μl / ￥4950

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安诺伦（北京）生物科技有限公司

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Cyclin E1 Antibody

品牌	Leading Biology	货号	APR00378G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Cyclin E1 Antibody.
分子量	47077 Da
细胞定位	Antigen Cellular Localization: Nucleus.
宿主	Rabbit
种属反应性	Human, Mouse, Rat, Pig, Bovine, Dog
靶点	Cyclin E1
亚型	Rabbit IgG
通用名	CCNE
基因ID	898
UniProt ID	P24864
功能	Essential for the control of the cell cycle at the G1/S (start) transition.
总结	Cyclin E1 is a regulatory subunit of the CDK2 kinase. It is central for the regulation of the G1/S transition and its abundance is tightly regulated throughout the cell cycle via ubiquitination. Cyclin E has been the subject of intense study in relation to tumorigenesis and cancer management and prognosis. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
形式	Liquid
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, IHC, IF, ICC
图像	Immunofluorescent analysis of Cyclin E1 staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ?C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Immunohistochemical analysis of Cyclin E1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Western blot analysis of Cyclin E1 expression in HEK293T (A), mouse brain (B), PC12 (C), SW626 (D), HeLa (E), K562 (F) whole cell lysates.
说明书	Product Datasheet Protocol
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